\u5ef6\u9577 (Extension)<\/li>\n<\/ol>\n\u5909\u6027 (Denaturation)<\/h3>\n
\u3053\u306e\u30b9\u30c6\u30c3\u30d7\u3067\u306f\u3001DNA\u306e\u4e8c\u91cd\u3089\u305b\u3093\u69cb\u9020\u304c\u9ad8\u6e29 (94\u00b0C – 98\u00b0C) \u306b\u3088\u3063\u3066\u89e3\u3051\u3066\u4e00\u672c\u9396\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n
\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0 (Annealing)<\/h3>\n
\u6b21\u306b\u3001\u6e29\u5ea6\u3092\u4f4e\u4e0b (50\u00b0C – 65\u00b0C) \u3055\u305b\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u304c\u5358\u4e00\u9396DNA\u306b\u7d50\u5408 (\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0) \u3057\u307e\u3059\u3002<\/p>\n
\u5ef6\u9577 (Extension)<\/h3>\n
\u6700\u5f8c\u306b\u3001\u6e29\u5ea6\u3092\u9069\u5ea6\u306a\u30ec\u30d9\u30eb (68\u00b0C – 72\u00b0C) \u306b\u4fdd\u3061\u3001DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u304c\u65b0\u3057\u3044DNA\u9396\u3092\u5408\u6210\u3057\u307e\u3059\u3002<\/p>\n
\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306e\u5f79\u5272<\/h2>\n
\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306f\u3001\u3053\u308c\u3089\u306e\u30b9\u30c6\u30c3\u30d7\u3054\u3068\u306b\u6e29\u5ea6\u3092\u7d20\u65e9\u304f\u6b63\u78ba\u306b\u5909\u66f4\u3059\u308b\u6a5f\u80fd\u3092\u6301\u3063\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u306b\u3088\u308a\u3001\u5404\u30b9\u30c6\u30c3\u30d7\u304c\u52b9\u7387\u7684\u306b\u9032\u884c\u3057\u3001\u76ee\u7684\u306eDNA\u304c\u52b9\u7387\u3088\u304f\u5897\u5e45\u3055\u308c\u307e\u3059\u3002<\/p>\n
\u6e29\u5ea6\u306e\u8fc5\u901f\u306a\u5909\u66f4<\/h3>\n
\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306f\u3001\u9ad8\u7cbe\u5ea6\u306a\u6e29\u5ea6\u5236\u5fa1\u30b7\u30b9\u30c6\u30e0\u3092\u7528\u3044\u3066\u3001\u6570\u79d2\u4ee5\u5185\u306b\u6e29\u5ea6\u3092\u5909\u66f4\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3053\u306e\u305f\u3081\u3001PCR\u30b5\u30a4\u30af\u30eb\u5168\u4f53\u304c\u77ed\u6642\u9593\u3067\u5b8c\u4e86\u3057\u307e\u3059\u3002<\/p>\n
\u5747\u4e00\u306a\u6e29\u5ea6\u5206\u5e03<\/h3>\n
\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306e\u53cd\u5fdc\u30c1\u30e5\u30fc\u30d6\u5185\u306e\u6e29\u5ea6\u304c\u5747\u4e00\u3067\u3042\u308b\u3053\u3068\u306f\u3001\u3059\u3079\u3066\u306eDNA\u5206\u5b50\u304c\u540c\u3058\u6761\u4ef6\u3067\u53cd\u5fdc\u3059\u308b\u305f\u3081\u306b\u91cd\u8981\u3067\u3059\u3002\u3053\u308c\u306b\u3088\u308a\u3001PCR\u306f\u9ad8\u3044\u518d\u73fe\u6027\u3092\u6301\u3061\u307e\u3059\u3002<\/p>\n
\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306e\u6280\u8853<\/h2>\n
\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306f\u4e3b\u306b\u4ee5\u4e0b\u306e\u6280\u8853\u3092\u4f7f\u7528\u3057\u3066\u3044\u307e\u3059\uff1a<\/p>\n
\n- \u30da\u30eb\u30c1\u30a7\u7d20\u5b50 (Peltier Element)\uff1a\u71b1\u96fb\u52b9\u679c\u3092\u5229\u7528\u3057\u3066\u8fc5\u901f\u306a\u52a0\u71b1\u3068\u51b7\u5374\u3092\u53ef\u80fd\u306b\u3057\u307e\u3059\u3002<\/li>\n
- \u9ad8\u7cbe\u5ea6\u30bb\u30f3\u30b5\u30fc\uff1a\u6e29\u5ea6\u3092\u6b63\u78ba\u306b\u8a08\u6e2c\u3057\u3001\u5fc5\u8981\u306a\u8abf\u6574\u3092\u884c\u3044\u307e\u3059\u3002<\/li>\n
- \u30d7\u30ed\u30b0\u30e9\u30e0\u53ef\u80fd\u306a\u5236\u5fa1\u30b7\u30b9\u30c6\u30e0\uff1a\u30e6\u30fc\u30b6\u30fc\u304c\u8a2d\u5b9a\u3057\u305f\u30b5\u30a4\u30af\u30eb\u3054\u3068\u306b\u6e29\u5ea6\u3092\u81ea\u52d5\u7684\u306b\u5909\u66f4\u3057\u307e\u3059\u3002<\/li>\n<\/ul>\n
\u307e\u3068\u3081<\/h2>\n
\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306f\u3001\u6e29\u5ea6\u3092\u7cbe\u5bc6\u306b\u5236\u5fa1\u3059\u308b\u3053\u3068\u3067PCR\u3092\u52b9\u7387\u7684\u306b\u4fc3\u9032\u3057\u307e\u3059\u3002\u3053\u308c\u306b\u3088\u308a\u3001\u533b\u5b66\u3001\u74b0\u5883\u79d1\u5b66\u3001\u6cd5\u533b\u5b66\u306a\u3069\u306e\u591a\u304f\u306e\u5206\u91ce\u3067\u6b20\u304b\u305b\u306a\u3044\u6280\u8853\u3068\u306a\u3063\u3066\u3044\u307e\u3059\u3002\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306e\u4f7f\u7528\u306b\u3088\u3063\u3066\u3001\u7814\u7a76\u8005\u306f\u8fc5\u901f\u304b\u3064\u6b63\u78ba\u306bDNA\u3092\u5897\u5e45\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"
\u300c\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u300d\u304cPCR\u306b\u304a\u3044\u3066\u3069\u306e\u3088\u3046\u306b\u6a5f\u80fd\u3057\u3001\u6e29\u5ea6\u3092\u5236\u5fa1\u3059\u308b\u3053\u3068\u3067DNA\u5897\u5e45\u3092\u52b9\u7387\u7684\u306b\u884c\u3046\u304b\u306b\u3064\u3044\u3066\u306e\u89e3\u8aac\u3002<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[125],"tags":[],"yoast_head":"\n
\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u304cPCR\u3092\u4fc3\u9032\u3059\u308b\u4ed5\u7d44\u307f<\/title>\n<meta name=\"description\" content=\"\u300c\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u300d\u304cPCR\u306b\u304a\u3044\u3066\u3069\u306e\u3088\u3046\u306b\u6a5f\u80fd\u3057\u3001\u6e29\u5ea6\u3092\u5236\u5fa1\u3059\u308b\u3053\u3068\u3067DNA\u5897\u5e45\u3092\u52b9\u7387\u7684\u306b\u884c\u3046\u304b\u306b\u3064\u3044\u3066\u306e\u89e3\u8aac\u3002\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermal-engineering.org\/ja\/\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u304cpcr\u3092\u4fc3\u9032\u3059\u308b\u4ed5\u7d44\u307f\/\" \/>\n<meta property=\"og:locale\" content=\"ja_JP\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" 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![\"\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u304cPCR\u3092\u4fc3\u9032\u3059\u308b\u4ed5\u7d44\u307f\"](\"https:\/\/www.thermal-engineering.org\/wp-content\/uploads\/2024\/06\/\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u304cPCR\u3092\u4fc3\u9032\u3059\u308b\u4ed5\u7d44\u307f.png\")
<\/p>\n