{"id":378362,"date":"2024-06-26T14:02:10","date_gmt":"2024-06-26T13:02:10","guid":{"rendered":"https:\/\/www.thermal-engineering.org\/pcr%e3%82%92%e5%ae%9f%e8%a1%8c%e3%81%99%e3%82%8b%e3%82%b5%e3%83%bc%e3%83%9e%e3%83%ab%e3%82%b5%e3%82%a4%e3%82%af%e3%83%a9%e3%83%bc%e3%81%ae%e4%bb%95%e7%b5%84%e3%81%bf\/"},"modified":"2024-06-26T14:02:10","modified_gmt":"2024-06-26T13:02:10","slug":"pcr%e3%82%92%e5%ae%9f%e8%a1%8c%e3%81%99%e3%82%8b%e3%82%b5%e3%83%bc%e3%83%9e%e3%83%ab%e3%82%b5%e3%82%a4%e3%82%af%e3%83%a9%e3%83%bc%e3%81%ae%e4%bb%95%e7%b5%84%e3%81%bf","status":"publish","type":"post","link":"https:\/\/www.thermal-engineering.org\/ja\/pcr%e3%82%92%e5%ae%9f%e8%a1%8c%e3%81%99%e3%82%8b%e3%82%b5%e3%83%bc%e3%83%9e%e3%83%ab%e3%82%b5%e3%82%a4%e3%82%af%e3%83%a9%e3%83%bc%e3%81%ae%e4%bb%95%e7%b5%84%e3%81%bf\/","title":{"rendered":"PCR\u3092\u5b9f\u884c\u3059\u308b\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306e\u4ed5\u7d44\u307f"},"content":{"rendered":"

\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306e\u4ed5\u7d44\u307f\u3092\u89e3\u8aac\u3002PCR\u6280\u8853\u306e\u6838\u5fc3\u3067\u3001DNA\u306e\u5909\u6027\u3001\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u3001\u4f38\u9577\u3092\u9ad8\u7cbe\u5ea6\u306b\u7e70\u308a\u8fd4\u3059\u88c5\u7f6e\u306e\u8a73\u7d30\u3002<\/p>\n

\"PCR\u3092\u5b9f\u884c\u3059\u308b\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306e\u4ed5\u7d44\u307f\"<\/p>\n

PCR\u3092\u5b9f\u884c\u3059\u308b\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306e\u4ed5\u7d44\u307f<\/h2>\n

PCR\uff08\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u9023\u9396\u53cd\u5fdc\uff09\u306f\u3001\u7279\u5b9a\u306eDNA\u65ad\u7247\u3092\u77ed\u6642\u9593\u3067\u5927\u91cf\u306b\u5897\u5e45\u3059\u308b\u305f\u3081\u306e\u6280\u8853\u3067\u3059\u3002\u3053\u306e\u6280\u8853\u306f\u3001\u907a\u4f1d\u5b50\u7814\u7a76\u3001\u533b\u5b66\u8a3a\u65ad\u3001\u6cd5\u533b\u5b66\u306a\u3069\u3055\u307e\u3056\u307e\u306a\u5206\u91ce\u3067\u5229\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002PCR\u3092\u884c\u3046\u305f\u3081\u306b\u306f\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u3068\u3044\u3046\u88c5\u7f6e\u304c\u4e0d\u53ef\u6b20\u3067\u3059\u3002\u3053\u306e\u8a18\u4e8b\u3067\u306f\u3001\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306e\u4ed5\u7d44\u307f\u306b\u3064\u3044\u3066\u8a73\u3057\u304f\u8aac\u660e\u3057\u307e\u3059\u3002<\/p>\n

\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u3068\u306f\uff1f<\/h2>\n

\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306f\u3001PCR\u53cd\u5fdc\u3092\u884c\u3046\u305f\u3081\u306e\u88c5\u7f6e\u3067\u3042\u308a\u3001\u7279\u5b9a\u306e\u6e29\u5ea6\u30b5\u30a4\u30af\u30eb\u3092\u6b63\u78ba\u306b\u8a2d\u5b9a\u3057\u3001\u7dad\u6301\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002PCR\u30d7\u30ed\u30bb\u30b9\u3067\u306f\u3001DNA\u306e\u5909\u6027\u3001\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u3001\u4f38\u9577\u3068\u3044\u30463\u3064\u306e\u4e3b\u8981\u306a\u30b9\u30c6\u30c3\u30d7\u3092\u7e70\u308a\u8fd4\u3057\u884c\u3044\u307e\u3059\u3002\u305d\u308c\u305e\u308c\u306e\u30b9\u30c6\u30c3\u30d7\u306b\u306f\u7570\u306a\u308b\u6e29\u5ea6\u3068\u6642\u9593\u304c\u5fc5\u8981\u3067\u3059\u3002<\/p>\n

\u6e29\u5ea6\u30b5\u30a4\u30af\u30eb\u306e\u57fa\u672c<\/h2>\n

\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306f\u3001\u4ee5\u4e0b\u306e\u6e29\u5ea6\u30b5\u30a4\u30af\u30eb\u3092\u7e70\u308a\u8fd4\u3059\u3053\u3068\u3067PCR\u53cd\u5fdc\u3092\u5b9f\u884c\u3057\u307e\u3059:<\/p>\n

<\/p>\n

  • \u5909\u6027 (Denaturation)<\/strong>: 95\u2103\u4ed8\u8fd1\u3067\u884c\u308f\u308c\u3001\u7d0415\u79d2\u301c30\u79d2\u9593\u6301\u7d9a\u3057\u307e\u3059\u3002\u3053\u306e\u9ad8\u6e29\u306b\u3088\u3063\u3066DNA\u4e8c\u672c\u9396\u304c\u4e00\u672c\u9396\u306b\u5206\u96e2\u3055\u308c\u307e\u3059\u3002<\/li>\n
  • \u30a2\u30cb\u30fc\u30ea\u30f3\u30b0 (Annealing)<\/strong>: 55\u301c65\u2103\u306e\u7bc4\u56f2\u3067\u884c\u308f\u308c\u3001\u7d0415\u79d2\u301c30\u79d2\u9593\u6301\u7d9a\u3057\u307e\u3059\u3002\u3053\u306e\u30b9\u30c6\u30c3\u30d7\u3067\u30d7\u30e9\u30a4\u30de\u30fc\u304cDNA\u306e\u7279\u5b9a\u90e8\u4f4d\u306b\u7d50\u5408\u3057\u307e\u3059\u3002<\/li>\n
  • \u4f38\u9577 (Extension\/Elongation)<\/strong>: 72\u2103\u3067\u884c\u308f\u308c\u3001\u6642\u9593\u306fDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u7a2e\u985e\u3084DNA\u306e\u9577\u3055\u306b\u3088\u308a\u307e\u3059\u304c\u3001\u4e00\u822c\u7684\u306b\u306f\u7d0430\u79d2\u301c1\u5206\u9593\u3067\u3059\u3002\u3053\u306e\u6bb5\u968e\u3067\u65b0\u3057\u3044DNA\u9396\u304c\u5408\u6210\u3055\u308c\u307e\u3059\u3002<\/li>\n

    <\/u1><\/p>\n

    \u3053\u308c\u3089\u306e\u30b5\u30a4\u30af\u30eb\u3092\u901a\u5e3820\u301c40\u56de\u7e70\u308a\u8fd4\u3057\u307e\u3059\u3002<\/p>\n

    \u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306e\u69cb\u9020<\/h2>\n

    \u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306f\u4e3b\u306b\u4ee5\u4e0b\u306e\u90e8\u5206\u304b\u3089\u69cb\u6210\u3055\u308c\u3066\u3044\u307e\u3059:<\/p>\n

    <\/p>\n

  • \u52a0\u71b1\/\u51b7\u5374\u30d6\u30ed\u30c3\u30af<\/strong>: \u30b5\u30f3\u30d7\u30eb\u3092\u4fdd\u6301\u3057\u3001\u6b63\u78ba\u306a\u6e29\u5ea6\u5236\u5fa1\u3092\u884c\u3046\u305f\u3081\u306e\u90e8\u5206\u3067\u3059\u3002<\/li>\n
  • \u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u30b7\u30b9\u30c6\u30e0<\/strong>: \u30d7\u30ed\u30b0\u30e9\u30e0\u53ef\u80fd\u306a\u6e29\u5ea6\u3068\u6642\u9593\u306e\u30d7\u30ed\u30c8\u30b3\u30eb\u3092\u8a2d\u5b9a\u3067\u304d\u308b\u30b7\u30b9\u30c6\u30e0\u3067\u3059\u3002<\/li>\n
  • \u30c7\u30a3\u30b9\u30d7\u30ec\u30a4\u3068\u30e6\u30fc\u30b6\u30fc\u30a4\u30f3\u30bf\u30fc\u30d5\u30a7\u30fc\u30b9<\/strong>: \u6e29\u5ea6\u30b5\u30a4\u30af\u30eb\u306e\u8a2d\u5b9a\u3084\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u306e\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u3092\u884c\u3046\u305f\u3081\u306e\u753b\u9762\u3068\u64cd\u4f5c\u30dc\u30bf\u30f3\u3067\u3059\u3002<\/li>\n

    <\/u1><\/p>\n

    \u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306e\u5fdc\u7528<\/h2>\n

    \u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306f\u975e\u5e38\u306b\u9ad8\u3044\u7cbe\u5ea6\u3067\u6e29\u5ea6\u3092\u5236\u5fa1\u3067\u304d\u308b\u305f\u3081\u3001\u4ee5\u4e0b\u306e\u3088\u3046\u306a\u591a\u304f\u306e\u5fdc\u7528\u304c\u53ef\u80fd\u3067\u3059:<\/p>\n

    <\/p>\n

  • \u907a\u4f1d\u5b50\u89e3\u6790<\/strong>: \u7279\u5b9a\u306e\u907a\u4f1d\u5b50\u3084\u5909\u7570\u306e\u691c\u51fa\u306b\u5229\u7528\u3055\u308c\u307e\u3059\u3002<\/li>\n
  • \u6cd5\u533b\u5b66<\/strong>: \u72af\u7f6a\u73fe\u5834\u3067\u63a1\u53d6\u3055\u308c\u305fDNA\u30b5\u30f3\u30d7\u30eb\u306e\u5206\u6790\u306b\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002<\/li>\n
  • \u611f\u67d3\u75c7\u8a3a\u65ad<\/strong>: \u7279\u5b9a\u306e\u30a6\u30a4\u30eb\u30b9\u3084\u7d30\u83cc\u306eDNA\/RNA\u3092\u5897\u5e45\u3057\u3066\u691c\u51fa\u3057\u307e\u3059\u3002<\/li>\n

    <\/u1><\/p>\n

    \u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306f\u3001PCR\u6280\u8853\u306e\u6838\u5fc3\u3068\u3057\u3066\u3001\u79d1\u5b66\u304a\u3088\u3073\u533b\u5b66\u7814\u7a76\u306e\u3055\u307e\u3056\u307e\u306a\u5206\u91ce\u3067\u6b20\u304b\u305b\u306a\u3044\u88c5\u7f6e\u3067\u3059\u3002\u3053\u306e\u6280\u8853\u304c\u3055\u3089\u306a\u308b\u9032\u5316\u3092\u9042\u3052\u308b\u3053\u3068\u3067\u3001\u672a\u6765\u306e\u533b\u7642\u3084\u79d1\u5b66\u306e\u53ef\u80fd\u6027\u304c\u5e83\u304c\u308b\u3053\u3068\u304c\u671f\u5f85\u3055\u308c\u307e\u3059\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

    \u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306e\u4ed5\u7d44\u307f\u3092\u89e3\u8aac\u3002PCR\u6280\u8853\u306e\u6838\u5fc3\u3067\u3001DNA\u306e\u5909\u6027\u3001\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u3001\u4f38\u9577\u3092\u9ad8\u7cbe\u5ea6\u306b\u7e70\u308a\u8fd4\u3059\u88c5\u7f6e\u306e\u8a73\u7d30\u3002<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[125],"tags":[],"yoast_head":"\nPCR\u3092\u5b9f\u884c\u3059\u308b\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306e\u4ed5\u7d44\u307f<\/title>\n<meta name=\"description\" content=\"\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306e\u4ed5\u7d44\u307f\u3092\u89e3\u8aac\u3002PCR\u6280\u8853\u306e\u6838\u5fc3\u3067\u3001DNA\u306e\u5909\u6027\u3001\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u3001\u4f38\u9577\u3092\u9ad8\u7cbe\u5ea6\u306b\u7e70\u308a\u8fd4\u3059\u88c5\u7f6e\u306e\u8a73\u7d30\u3002\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermal-engineering.org\/ja\/pcr\u3092\u5b9f\u884c\u3059\u308b\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306e\u4ed5\u7d44\u307f\/\" \/>\n<meta property=\"og:locale\" content=\"ja_JP\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"PCR\u3092\u5b9f\u884c\u3059\u308b\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306e\u4ed5\u7d44\u307f\" \/>\n<meta property=\"og:description\" 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