{"id":383180,"date":"2024-06-26T15:07:49","date_gmt":"2024-06-26T14:07:49","guid":{"rendered":"https:\/\/www.thermal-engineering.org\/%e7%ad%89%e6%b8%a9%e6%bb%b4%e5%ae%9a%e7%86%b1%e9%87%8f%e6%b8%ac%e5%ae%9a%e6%b3%95-itc\/"},"modified":"2024-06-26T15:07:49","modified_gmt":"2024-06-26T14:07:49","slug":"%e7%ad%89%e6%b8%a9%e6%bb%b4%e5%ae%9a%e7%86%b1%e9%87%8f%e6%b8%ac%e5%ae%9a%e6%b3%95-itc","status":"publish","type":"post","link":"https:\/\/www.thermal-engineering.org\/ja\/%e7%ad%89%e6%b8%a9%e6%bb%b4%e5%ae%9a%e7%86%b1%e9%87%8f%e6%b8%ac%e5%ae%9a%e6%b3%95-itc\/","title":{"rendered":"\u7b49\u6e29\u6ef4\u5b9a\u71b1\u91cf\u6e2c\u5b9a\u6cd5 (ITC)"},"content":{"rendered":"

\u7b49\u6e29\u6ef4\u5b9a\u71b1\u91cf\u6e2c\u5b9a\u6cd5 (ITC) \u306f\u3001\u5206\u5b50\u9593\u76f8\u4e92\u4f5c\u7528\u3092\u89e3\u6790\u3057\u3001\u7d50\u5408\u5b9a\u6570\u3084\u53cd\u5fdc\u30a8\u30f3\u30bf\u30eb\u30d4\u30fc\u5909\u5316\u306a\u3069\u306e\u71b1\u529b\u5b66\u7684\u30d1\u30e9\u30e1\u30fc\u30bf\u3092\u7b97\u51fa\u3059\u308b\u6280\u8853\u3067\u3059\u3002<\/p>\n

\"\u7b49\u6e29\u6ef4\u5b9a\u71b1\u91cf\u6e2c\u5b9a\u6cd5<\/p>\n

\u7b49\u6e29\u6ef4\u5b9a\u71b1\u91cf\u6e2c\u5b9a\u6cd5 (ITC)<\/h2>\n

\u7b49\u6e29\u6ef4\u5b9a\u71b1\u91cf\u6e2c\u5b9a\u6cd5\uff08Isothermal Titration Calorimetry\u3001ITC\uff09\u306f\u3001\u5316\u5b66\u304a\u3088\u3073\u751f\u7269\u5b66\u306b\u304a\u3044\u3066\u5206\u5b50\u9593\u76f8\u4e92\u4f5c\u7528\u3092\u7814\u7a76\u3059\u308b\u5f37\u529b\u306a\u6280\u8853\u3067\u3059\u3002\u3053\u306e\u65b9\u6cd5\u3067\u306f\u3001\u6ef4\u5b9a\u904e\u7a0b\u306b\u3088\u3063\u3066\u767a\u751f\u3059\u308b\u71b1\u3092\u6e2c\u5b9a\u3057\u3001\u71b1\u529b\u5b66\u7684\u30d1\u30e9\u30e1\u30fc\u30bf\u3092\u5c0e\u51fa\u3057\u307e\u3059\u3002\u5177\u4f53\u7684\u306b\u306f\u3001\u7d50\u5408\u5b9a\u6570 (K)\u3001\u53cd\u5fdc\u30a8\u30f3\u30bf\u30eb\u30d4\u30fc\u5909\u5316 (\u0394H)\u3001\u53cd\u5fdc\u30a8\u30f3\u30c8\u30ed\u30d4\u30fc\u5909\u5316 (\u0394S)\u3001\u304a\u3088\u3073\u53cd\u5fdc\u306e\u30ae\u30d6\u30b9\u81ea\u7531\u30a8\u30cd\u30eb\u30ae\u30fc (\u0394G) \u304c\u8a08\u7b97\u3067\u304d\u307e\u3059\u3002<\/p>\n

ITC\u306e\u57fa\u672c\u539f\u7406<\/h2>\n

\u7b49\u6e29\u6ef4\u5b9a\u71b1\u91cf\u6e2c\u5b9a\u6cd5\u306f\u3001\u6b21\u306e\u3088\u3046\u306a\u624b\u9806\u3067\u884c\u308f\u308c\u307e\u3059\u3002<\/p>\n

    \n
  1. \u6ef4\u5b9a\u30bb\u30eb\u306b\u6eb6\u6db2\u3092\u30bb\u30c3\u30c8\u3057\u307e\u3059\u3002<\/li>\n
  2. \u5fae\u5c0f\u306a\u4f53\u7a4d\u306e\u6ef4\u5b9a\u5264\u3092\u9806\u6b21\u8ffd\u52a0\u3057\u307e\u3059\u3002<\/li>\n
  3. \u8ffd\u52a0\u3054\u3068\u306b\u767a\u751f\u3059\u308b\u71b1\u3092\u6e2c\u5b9a\u3057\u3001\u5438\u71b1\u307e\u305f\u306f\u653e\u71b1\u30d7\u30ed\u30d5\u30a1\u30a4\u30eb\u3092\u8a18\u9332\u3057\u307e\u3059\u3002<\/li>\n
  4. \u3053\u308c\u3089\u306e\u30c7\u30fc\u30bf\u304b\u3089\u3001\u7d50\u5408\u53cd\u5fdc\u306e\u71b1\u529b\u5b66\u7684\u30d1\u30e9\u30e1\u30fc\u30bf\u3092\u8a08\u7b97\u3057\u307e\u3059\u3002<\/li>\n<\/ol>\n

    \u3053\u306e\u65b9\u6cd5\u306f\u3001\u5316\u5408\u7269\u9593\u306e\u76f8\u4e92\u4f5c\u7528\u3092\u8a73\u7d30\u306b\u89e3\u6790\u3059\u308b\u305f\u3081\u306b\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002ITC\u306f\u975e\u6a19\u8b58\u6cd5\u3067\u3042\u308a\u3001\u8a66\u6599\u306e\u6539\u5909\u3084\u6a19\u8b58\u4ed8\u304c\u4e0d\u8981\u3067\u3059\u3002<\/p>\n

    \u71b1\u529b\u5b66\u7684\u30d1\u30e9\u30e1\u30fc\u30bf<\/h2>\n

    ITC\u306b\u3088\u3063\u3066\u5f97\u3089\u308c\u308b\u4e3b\u8981\u306a\u71b1\u529b\u5b66\u7684\u30d1\u30e9\u30e1\u30fc\u30bf\u306b\u3064\u3044\u3066\u8aac\u660e\u3057\u307e\u3059\u3002<\/p>\n

      \n
    • \u7d50\u5408\u5b9a\u6570 (K)<\/b>: \u53cd\u5fdc\u306e\u5e73\u8861\u5b9a\u6570\u3067\u3059\u3002\u5c0f\u3055\u3044K\u5024\u306f\u5f31\u3044\u7d50\u5408\u3001\u5927\u304d\u3044K\u5024\u306f\u5f37\u3044\u7d50\u5408\u3092\u793a\u3057\u307e\u3059\u3002<\/li>\n
    • \u53cd\u5fdc\u30a8\u30f3\u30bf\u30eb\u30d4\u30fc\u5909\u5316 (\u0394H)<\/b>: \u53cd\u5fdc\u306b\u4f34\u3046\u71b1\u5909\u5316\u3067\u3059\u3002\u6b63\u8ca0\u306e\u7b26\u53f7\u306b\u3088\u3063\u3066\u5438\u71b1\u53cd\u5fdc\u304b\u653e\u71b1\u53cd\u5fdc\u304b\u304c\u5206\u304b\u308a\u307e\u3059\u3002<\/li>\n
    • \u53cd\u5fdc\u30a8\u30f3\u30c8\u30ed\u30d4\u30fc\u5909\u5316 (\u0394S)<\/b>: \u53cd\u5fdc\u306e\u7121\u79e9\u5e8f\u3055\u306e\u5909\u5316\u3092\u793a\u3057\u307e\u3059\u3002\u0394H\u304a\u3088\u3073\u0394G\u3068\u4f75\u305b\u3066\u8a08\u7b97\u3055\u308c\u307e\u3059\u3002<\/li>\n
    • \u53cd\u5fdc\u306e\u30ae\u30d6\u30b9\u81ea\u7531\u30a8\u30cd\u30eb\u30ae\u30fc (\u0394G)<\/b>: \u53cd\u5fdc\u306e\u81ea\u767a\u6027\u3092\u793a\u3057\u307e\u3059\u3002\u5f0f\u306f\u6b21\u306e\u3088\u3046\u306b\u306a\u308a\u307e\u3059\u3002<\/li>\n<\/ul>\n

      \\(\\Delta G = \\Delta H – T \\Delta S\\)<\/p>\n

      \u5fdc\u7528\u4f8b<\/h2>\n

      ITC\u306f\u591a\u304f\u306e\u79d1\u5b66\u5206\u91ce\u3067\u5fdc\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u4ee5\u4e0b\u306b\u3044\u304f\u3064\u304b\u306e\u4f8b\u3092\u793a\u3057\u307e\u3059\u3002<\/p>\n

        \n
      • \u9175\u7d20-\u57fa\u8cea\u76f8\u4e92\u4f5c\u7528<\/b>: \u9175\u7d20\u3068\u57fa\u8cea\u3068\u306e\u7d50\u5408\u306e\u5f37\u3055\u3084\u3001\u7d50\u5408\u306e\u71b1\u529b\u5b66\u7684\u7279\u6027\u3092\u6e2c\u5b9a\u3057\u307e\u3059\u3002<\/li>\n
      • \u85ac\u7269\u8a55\u4fa1<\/b>: \u65b0\u85ac\u5019\u88dc\u5316\u5408\u7269\u306e\u6a19\u7684\u7d50\u5408\u7279\u6027\u3092\u8a55\u4fa1\u3057\u3001\u85ac\u52b9\u3092\u4e88\u6e2c\u3057\u307e\u3059\u3002<\/li>\n
      • \u30d0\u30a4\u30aa\u30bb\u30f3\u30b5\u30fc\u958b\u767a<\/b>: \u5206\u5b50\u8b58\u5225\u8981\u7d20\u306e\u7279\u6027\u8a55\u4fa1\u306b\u3088\u308a\u3001\u30bb\u30f3\u30b5\u30fc\u306e\u611f\u5ea6\u3068\u9078\u629e\u6027\u3092\u5411\u4e0a\u3055\u305b\u307e\u3059\u3002<\/li>\n<\/ul>\n

        \u7b49\u6e29\u6ef4\u5b9a\u71b1\u91cf\u6e2c\u5b9a\u6cd5 (ITC) \u306f\u3001\u5206\u5b50\u9593\u76f8\u4e92\u4f5c\u7528\u3092\u7406\u89e3\u3059\u308b\u305f\u3081\u306e\u8cb4\u91cd\u306a\u30c4\u30fc\u30eb\u3067\u3042\u308a\u3001\u79d1\u5b66\u7814\u7a76\u304b\u3089\u88fd\u85ac\u958b\u767a\u307e\u3067\u5e45\u5e83\u3044\u5206\u91ce\u3067\u5fdc\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u305d\u306e\u975e\u4fb5\u8972\u6027\u3068\u9ad8\u611f\u5ea6\u306f\u3001\u7814\u7a76\u8005\u306b\u3068\u3063\u3066\u6975\u3081\u3066\u6709\u7528\u3067\u3059\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

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