{"id":331712,"date":"2024-06-19T21:10:06","date_gmt":"2024-06-19T20:10:06","guid":{"rendered":"https:\/\/www.thermal-engineering.org\/%ec%97%b4%ec%88%9c%ed%99%98%ea%b8%b0%ea%b0%80-dna-%eb%b3%b5%ec%a0%9c%ec%97%90-%eb%8f%84%ec%9b%80%ec%9d%84-%ec%a3%bc%eb%8a%94-%eb%b0%a9%eb%b2%95\/"},"modified":"2024-06-19T21:10:06","modified_gmt":"2024-06-19T20:10:06","slug":"%ec%97%b4%ec%88%9c%ed%99%98%ea%b8%b0%ea%b0%80-dna-%eb%b3%b5%ec%a0%9c%ec%97%90-%eb%8f%84%ec%9b%80%ec%9d%84-%ec%a3%bc%eb%8a%94-%eb%b0%a9%eb%b2%95","status":"publish","type":"post","link":"https:\/\/www.thermal-engineering.org\/ko\/%ec%97%b4%ec%88%9c%ed%99%98%ea%b8%b0%ea%b0%80-dna-%eb%b3%b5%ec%a0%9c%ec%97%90-%eb%8f%84%ec%9b%80%ec%9d%84-%ec%a3%bc%eb%8a%94-%eb%b0%a9%eb%b2%95\/","title":{"rendered":"\uc5f4\uc21c\ud658\uae30\uac00 DNA \ubcf5\uc81c\uc5d0 \ub3c4\uc6c0\uc744 \uc8fc\ub294 \ubc29\ubc95"},"content":{"rendered":"<p class=\"sidekick\">PCR \uacfc\uc815\uc5d0\uc11c DNA\ub97c \ud6a8\uc728\uc801\uc73c\ub85c \uc99d\ud3ed\uc2dc\ud0a4\ub294 \uc5f4\uc21c\ud658\uae30\uc758 \uc5ed\ud560\uacfc \uc911\uc694\uc131\uc5d0 \ub300\ud574 \uc124\uba85\ud558\ub294 \uae00\uc785\ub2c8\ub2e4. \uc0dd\uba85\uacf5\ud559 \uc5f0\uad6c\uc5d0\uc11c \ud544\uc218\uc801\uc778 \ub3c4\uad6c\uc785\ub2c8\ub2e4.<\/p>\n<p><img src=\"https:\/\/www.thermal-engineering.org\/wp-content\/uploads\/2024\/06\/\uc5f4\uc21c\ud658\uae30\uac00_DNA_\ubcf5\uc81c\uc5d0_\ub3c4\uc6c0\uc744_\uc8fc\ub294_\ubc29\ubc95.png\" alt=\"\uc5f4\uc21c\ud658\uae30\uac00 DNA \ubcf5\uc81c\uc5d0 \ub3c4\uc6c0\uc744 \uc8fc\ub294 \ubc29\ubc95\" style=\"display: block; margin-left: auto; margin-right: auto;\"\/><\/p>\n<h2>\uc5f4\uc21c\ud658\uae30\uac00 DNA \ubcf5\uc81c\uc5d0 \ub3c4\uc6c0\uc744 \uc8fc\ub294 \ubc29\ubc95<\/h2>\n<p>\uc5f4\uc21c\ud658\uae30, \ub610\ub294 \uc5f4\uc21c\ud658 \uae30\uacc4(thermocycler)\ub294 \uc8fc\ub85c \uc911\ud569\ud6a8\uc18c \uc5f0\uc1c4 \ubc18\uc751(PCR, Polymerase Chain Reaction)\uc5d0\uc11c \uc0ac\uc6a9\ub418\ub294 \uae30\uacc4\uc785\ub2c8\ub2e4. PCR\uc740 DNA\uc758 \ud2b9\uc815 \ubd80\ubd84\uc744 \ub300\ub7c9\uc73c\ub85c \uc99d\ud3ed\uc2dc\ud0a4\uae30 \uc704\ud574 \ud754\ud788 \uc0ac\uc6a9\ub418\ub294 \uc0dd\uba85\uacf5\ud559 \uae30\uc220\uc785\ub2c8\ub2e4. \uc774 \uae30\uc0ac\uc5d0\uc11c\ub294 \uc5f4\uc21c\ud658\uae30\uac00 \uc5b4\ub5bb\uac8c DNA \ubcf5\uc81c\uc5d0 \ub3c4\uc6c0\uc744 \uc8fc\ub294\uc9c0\uc5d0 \ub300\ud574 \uc124\uba85\ud558\uaca0\uc2b5\ub2c8\ub2e4.<\/p>\n<h2>\uc911\ud569\ud6a8\uc18c \uc5f0\uc1c4 \ubc18\uc751(PCR)<\/h2>\n<p>PCR\uc740 \uc0dd\ud654\ud559\uc801 \uae30\uc220\ub85c, \uc801\uc740 \uc591\uc758 DNA\ub97c \uc2e0\uc18d\ud558\uac8c \uc99d\ud3ed\uc2dc\ucf1c \ubd84\uc11d \ubc0f \uc5f0\uad6c\ub97c \uac00\ub2a5\ud558\uac8c \ud569\ub2c8\ub2e4. PCR \uacfc\uc815\uc740 \ud06c\uac8c \uc138 \uac00\uc9c0 \ub2e8\uacc4\ub97c \ud3ec\ud568\ud558\uba70, \uac01\uac01\uc758 \ub2e8\uacc4\ub294 \ud2b9\uc815 \uc628\ub3c4\uc5d0\uc11c \uc218\ud589\ub429\ub2c8\ub2e4:<\/p>\n<ul>\n<li>\ubcc0\uc131 (Denaturation): DNA \uc774\uc911 \ub098\uc120\uc744 \ub2e8\uc77c \uac00\ub2e5\uc73c\ub85c \ubd84\ub9ac\uc2dc\ud0a4\uae30 \uc704\ud574 \uc57d 94-98\u00b0C\uc758 \uc628\ub3c4\ub85c \uac00\uc5f4\ud569\ub2c8\ub2e4.<\/li>\n<li>\uacb0\ud569 (Annealing): \ud504\ub77c\uc774\uba38\uac00 \ub2e8\uc77c \uac00\ub2e5 DNA\uc5d0 \uacb0\ud569\ud558\ub3c4\ub85d \uc57d 50-65\u00b0C\ub85c \ub0c9\uac01\ud569\ub2c8\ub2e4.<\/li>\n<li>\uc5f0\uc7a5 (Extension): DNA \uc911\ud569\ud6a8\uc18c\uac00 \uc0c8\ub85c\uc6b4 DNA \uac00\ub2e5\uc744 \ud569\uc131\ud558\ub3c4\ub85d \uc57d 72\u00b0C\ub85c \uc628\ub3c4\ub97c \uc870\uc808\ud569\ub2c8\ub2e4.<\/li>\n<\/ul>\n<p>\uc774 \uc138 \uac00\uc9c0 \ub2e8\uacc4\ub294 \uc5ec\ub7ec \ubc88 \ubc18\ubcf5\ub418\uba70, \uc774\ub97c \ud1b5\ud574 \uc6d0\ud558\ub294 DNA \ub2e8\ud3b8\uc744 \uc218\ubc31\ub9cc \ubc30\ub85c \uc99d\ud3ed\ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/p>\n<h2>\uc5f4\uc21c\ud658\uae30\uc758 \uc5ed\ud560<\/h2>\n<p>\uc5f4\uc21c\ud658\uae30\ub294 \uc0c1\uae30 \uc138 \uac00\uc9c0 \ub2e8\uacc4\ub97c \uc790\ub3d9\uc73c\ub85c \ubc18\ubcf5 \uc2e4\ud589\ud560 \uc218 \uc788\ub294 \uae30\uacc4\uc785\ub2c8\ub2e4. \uc774\ub97c \ud1b5\ud574 \uc5f0\uad6c\uc790\ub4e4\uc774 \uc815\ud655\ud558\uace0 \uc2e0\uc18d\ud558\uac8c \ub9ce\uc740 \uc591\uc758 DNA\ub97c \uc99d\ud3ed\ud560 \uc218 \uc788\uac8c \ub429\ub2c8\ub2e4. \uc5f4\uc21c\ud658\uae30\ub294 \uc815\ud655\ud55c \uc628\ub3c4 \uc870\uc808\uacfc \uc2dc\uac04\uc744 \ud1b5\ud574 \ub2e4\uc74c\uacfc \uac19\uc740 \uc5ed\ud560\uc744 \uc218\ud589\ud569\ub2c8\ub2e4:<\/p>\n<ol>\n<li>\uc628\ub3c4 \uc81c\uc5b4: \ubcc0\uc131, \uacb0\ud569, \uc5f0\uc7a5\uc758 \uc815\ud655\ud55c \uc628\ub3c4\ub97c \uc81c\uc5b4\ud558\uc5ec \uac01 \ub2e8\uacc4\uac00 \ucd5c\uc801\uc758 \uc870\uac74\uc5d0\uc11c \uc218\ud589\ub418\ub3c4\ub85d \ud569\ub2c8\ub2e4.<\/li>\n<li>\uc2dc\uac04 \uc81c\uc5b4: \uac01 \ub2e8\uacc4\uac00 \uc77c\uc815\ud55c \uc2dc\uac04 \ub3d9\uc548 \uc720\uc9c0\ub418\ub3c4\ub85d \ud558\uc5ec DNA \uc99d\ud3ed \uacfc\uc815\uc774 \ud6a8\uc728\uc801\uc73c\ub85c \uc9c4\ud589\ub418\uac8c \ud569\ub2c8\ub2e4.<\/li>\n<li>\uc790\ub3d9\ud654: \ubc18\ubcf5\ub418\ub294 \uc628\ub3c4 \ubcc0\ud654\ub97c \uc790\ub3d9\uc73c\ub85c \uad6c\ud604\ud558\uc5ec, \ubc18\ubcf5\uc801\uc778 \uc791\uc5c5\uc744 \uc790\ub3d9\ud654\ud569\ub2c8\ub2e4.<\/li>\n<\/ol>\n<h2>\uc911\uc694\ud55c \uac1c\ub150 \ubc0f \uc751\uc6a9<\/h2>\n<p>PCR \ubc0f \uc5f4\uc21c\ud658\uae30\ub294 \uc720\uc804\uc790 \uc5f0\uad6c, \uc9c8\ubcd1 \uc9c4\ub2e8, \ubc95\uc758\ud559 \ub4f1 \ub2e4\uc591\ud55c \ubd84\uc57c\uc5d0\uc11c \uc911\uc694\ud55c \uc5ed\ud560\uc744 \ud569\ub2c8\ub2e4. \uc608\ub97c \ub4e4\uc5b4, COVID-19 \ubc14\uc774\ub7ec\uc2a4\uc758 \uc720\uc804\uc790 \uac80\ucd9c\uc744 \uc704\ud55c RT-PCR \uac80\uc0ac\ub294 \uc5f4\uc21c\ud658\uae30\ub97c \uc0ac\uc6a9\ud558\uc5ec \ubc14\uc774\ub7ec\uc2a4 RNA\ub97c \uc99d\ud3ed\uc2dc\ud0b5\ub2c8\ub2e4.<\/p>\n<p>\ub610\ud55c, \uc911\ud569\ud6a8\uc18c \uc5f0\uc1c4 \ubc18\uc751\uc740 \uc2e0\uc18d\ud558\uace0 \uc815\ud655\ud55c DNA \ubd84\uc11d\uc744 \uac00\ub2a5\ud558\uac8c \ud558\uc5ec, \uc9c4\ud654 \uc5f0\uad6c, \uc720\uc804\uc790 \ubcc0\ud615, \uc0dd\ubb3c\ud559\uc801 \uc5f0\uad6c \ub4f1 \uc5ec\ub7ec \uc751\uc6a9 \ubd84\uc57c\uc5d0\uc11c \ub110\ub9ac \uc0ac\uc6a9\ub418\uace0 \uc788\uc2b5\ub2c8\ub2e4.<\/p>\n<h2>\uacb0\ub860<\/h2>\n<p>\uc5f4\uc21c\ud658\uae30\ub294 DNA \ubcf5\uc81c\uc640 \uc99d\ud3ed\uc758 \ud575\uc2ec \ub3c4\uad6c\ub85c, \ub2e4\uc591\ud55c \uacfc\ud559\uc801 \uc5f0\uad6c\uc640 \uc2e4\ud5d8\uc5d0\uc11c \ud544\uc218\uc801\uc778 \uae30\uacc4\uc785\ub2c8\ub2e4. \uc815\ud655\ud55c \uc628\ub3c4 \ubc0f \uc2dc\uac04 \uc870\uc808\uc744 \ud1b5\ud574 \ubcf5\uc7a1\ud55c PCR \uacfc\uc815\uc744 \uc790\ub3d9\ud654\ud558\uace0, \uc774\ub97c \ud1b5\ud574 \uc0dd\uba85\uacf5\ud559 \uc5f0\uad6c\uc758 \ud6a8\uc728\uc131\uacfc \uc815\ud655\uc131\uc744 \ub192\uc5ec\uc90d\ub2c8\ub2e4. \uc5f4\uc21c\ud658\uae30\uc758 \uae30\uc220\uc801 \ubc1c\uc804\uc740 \uc55e\uc73c\ub85c\ub3c4 \uc0dd\uba85\uacfc\ud559 \ubd84\uc57c\uc758 \uc911\uc694\ud55c \ud601\uc2e0\uc744 \uc774\ub04c\uc5b4\ub0bc \uac83\uc785\ub2c8\ub2e4.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>PCR \uacfc\uc815\uc5d0\uc11c DNA\ub97c \ud6a8\uc728\uc801\uc73c\ub85c \uc99d\ud3ed\uc2dc\ud0a4\ub294 \uc5f4\uc21c\ud658\uae30\uc758 \uc5ed\ud560\uacfc \uc911\uc694\uc131\uc5d0 \ub300\ud574 \uc124\uba85\ud558\ub294 \uae00\uc785\ub2c8\ub2e4. \uc0dd\uba85\uacf5\ud559 \uc5f0\uad6c\uc5d0\uc11c \ud544\uc218\uc801\uc778 \ub3c4\uad6c\uc785\ub2c8\ub2e4.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[124],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v15.4 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>\uc5f4\uc21c\ud658\uae30\uac00 DNA \ubcf5\uc81c\uc5d0 \ub3c4\uc6c0\uc744 \uc8fc\ub294 \ubc29\ubc95<\/title>\n<meta name=\"description\" content=\"PCR \uacfc\uc815\uc5d0\uc11c DNA\ub97c \ud6a8\uc728\uc801\uc73c\ub85c \uc99d\ud3ed\uc2dc\ud0a4\ub294 \uc5f4\uc21c\ud658\uae30\uc758 \uc5ed\ud560\uacfc \uc911\uc694\uc131\uc5d0 \ub300\ud574 \uc124\uba85\ud558\ub294 \uae00\uc785\ub2c8\ub2e4. \uc0dd\uba85\uacf5\ud559 \uc5f0\uad6c\uc5d0\uc11c \ud544\uc218\uc801\uc778 \ub3c4\uad6c\uc785\ub2c8\ub2e4.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermal-engineering.org\/ko\/\uc5f4\uc21c\ud658\uae30\uac00-dna-\ubcf5\uc81c\uc5d0-\ub3c4\uc6c0\uc744-\uc8fc\ub294-\ubc29\ubc95\/\" \/>\n<meta property=\"og:locale\" content=\"ko_KR\" \/>\n<meta property=\"og:type\" 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\\ubc29\\ubc95\"},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.thermal-engineering.org\/ko\/%ec%97%b4%ec%88%9c%ed%99%98%ea%b8%b0%ea%b0%80-dna-%eb%b3%b5%ec%a0%9c%ec%97%90-%eb%8f%84%ec%9b%80%ec%9d%84-%ec%a3%bc%eb%8a%94-%eb%b0%a9%eb%b2%95\/#webpage\",\"url\":\"https:\/\/www.thermal-engineering.org\/ko\/%ec%97%b4%ec%88%9c%ed%99%98%ea%b8%b0%ea%b0%80-dna-%eb%b3%b5%ec%a0%9c%ec%97%90-%eb%8f%84%ec%9b%80%ec%9d%84-%ec%a3%bc%eb%8a%94-%eb%b0%a9%eb%b2%95\/\",\"name\":\"\\uc5f4\\uc21c\\ud658\\uae30\\uac00 DNA \\ubcf5\\uc81c\\uc5d0 \\ub3c4\\uc6c0\\uc744 \\uc8fc\\ub294 \\ubc29\\ubc95\",\"isPartOf\":{\"@id\":\"https:\/\/www.thermal-engineering.org\/fr\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.thermal-engineering.org\/ko\/%ec%97%b4%ec%88%9c%ed%99%98%ea%b8%b0%ea%b0%80-dna-%eb%b3%b5%ec%a0%9c%ec%97%90-%eb%8f%84%ec%9b%80%ec%9d%84-%ec%a3%bc%eb%8a%94-%eb%b0%a9%eb%b2%95\/#primaryimage\"},\"datePublished\":\"2024-06-19T20:10:06+00:00\",\"dateModified\":\"2024-06-19T20:10:06+00:00\",\"author\":{\"@id\":\"https:\/\/www.thermal-engineering.org\/fr\/#\/schema\/person\/e8c544db9afedaec8574d6464f9398bb\"},\"description\":\"PCR \\uacfc\\uc815\\uc5d0\\uc11c DNA\\ub97c \\ud6a8\\uc728\\uc801\\uc73c\\ub85c \\uc99d\\ud3ed\\uc2dc\\ud0a4\\ub294 \\uc5f4\\uc21c\\ud658\\uae30\\uc758 \\uc5ed\\ud560\\uacfc \\uc911\\uc694\\uc131\\uc5d0 \\ub300\\ud574 \\uc124\\uba85\\ud558\\ub294 \\uae00\\uc785\\ub2c8\\ub2e4. \\uc0dd\\uba85\\uacf5\\ud559 \\uc5f0\\uad6c\\uc5d0\\uc11c \\ud544\\uc218\\uc801\\uc778 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