{"id":333824,"date":"2024-06-19T21:34:03","date_gmt":"2024-06-19T20:34:03","guid":{"rendered":"https:\/\/www.thermal-engineering.org\/%ec%97%b4-%ec%88%9c%ed%99%98-%ec%9e%a5%ec%b9%98%ea%b0%80-pcr%ec%9d%84-%ec%88%98%ed%96%89%ed%95%98%eb%8a%94-%eb%b0%a9%eb%b2%95\/"},"modified":"2024-06-19T21:34:03","modified_gmt":"2024-06-19T20:34:03","slug":"%ec%97%b4-%ec%88%9c%ed%99%98-%ec%9e%a5%ec%b9%98%ea%b0%80-pcr%ec%9d%84-%ec%88%98%ed%96%89%ed%95%98%eb%8a%94-%eb%b0%a9%eb%b2%95","status":"publish","type":"post","link":"https:\/\/www.thermal-engineering.org\/ko\/%ec%97%b4-%ec%88%9c%ed%99%98-%ec%9e%a5%ec%b9%98%ea%b0%80-pcr%ec%9d%84-%ec%88%98%ed%96%89%ed%95%98%eb%8a%94-%eb%b0%a9%eb%b2%95\/","title":{"rendered":"\uc5f4 \uc21c\ud658 \uc7a5\uce58\uac00 PCR\uc744 \uc218\ud589\ud558\ub294 \ubc29\ubc95"},"content":{"rendered":"

\uc5f4 \uc21c\ud658 \uc7a5\uce58\ub294 PCR \uc218\ud589 \uacfc\uc815\uc5d0\uc11c DNA \ubcf5\uc81c\ub97c \uc704\ud574 \uc138 \ub2e8\uacc4 \uc628\ub3c4 \ubcc0\ud654\ub97c \uc815\ubc00\ud558\uac8c \uc81c\uc5b4\ud558\ub294 \uc911\uc694\ud55c \uc2e4\ud5d8 \uae30\uae30\uc785\ub2c8\ub2e4.<\/p>\n

\"\uc5f4<\/p>\n

\uc5f4 \uc21c\ud658 \uc7a5\uce58\uac00 PCR\uc744 \uc218\ud589\ud558\ub294 \ubc29\ubc95<\/h2>\n

\uc911\ud569\ud6a8\uc18c \uc5f0\uc1c4 \ubc18\uc751(PCR, Polymerase Chain Reaction)\uc740 \uc720\uc804\uc790 \uc5f0\uad6c\ub098 \uc9c8\ubcd1 \uc9c4\ub2e8\uc5d0 \ub110\ub9ac \uc0ac\uc6a9\ub418\ub294 \uc911\uc694\ud55c \uc2e4\ud5d8 \uae30\ubc95\uc785\ub2c8\ub2e4. \uc774 \uacfc\uc815\uc5d0\uc11c \uc5f4 \uc21c\ud658 \uc7a5\uce58(thermal cycler)\ub294 \ub9e4\uc6b0 \uc911\uc694\ud55c \uc5ed\ud560\uc744 \ud569\ub2c8\ub2e4. \uc774 \uc7a5\uce58\ub294 DNA \ubcf5\uc81c\ub97c \uc704\ud574 \ud544\uc694\ud55c \uc628\ub3c4 \ubcc0\ud654\ub97c \uc815\ud655\ud558\uac8c \uc81c\uc5b4\ud569\ub2c8\ub2e4. \uc774\ubc88 \uae30\uc0ac\uc5d0\uc11c\ub294 \uc5f4 \uc21c\ud658 \uc7a5\uce58\uac00 PCR\uc744 \uc218\ud589\ud558\ub294 \ubc29\ubc95\uc5d0 \ub300\ud574 \uc124\uba85\ud558\uaca0\uc2b5\ub2c8\ub2e4.<\/p>\n

PCR\uc758 \uae30\ubcf8 \uc6d0\ub9ac<\/h2>\n

PCR\uc740 \ub2e4\uc74c \uc138 \ub2e8\uacc4\ub85c \uc774\ub8e8\uc5b4\uc9d1\ub2c8\ub2e4:<\/p>\n

<\/p>\n

  • \ubcc0\uc131 (Denaturation): 94-98\u00b0C<\/li>\n
  • \uc811\ud569 (Annealing): 50-65\u00b0C<\/li>\n
  • \uc2e0\uc7a5 (Extension): 72\u00b0C<\/li>\n

    <\/u1><\/p>\n

    \uc5f4 \uc21c\ud658 \uc7a5\uce58\uc758 \uc5ed\ud560<\/h2>\n

    \uc5f4 \uc21c\ud658 \uc7a5\uce58\ub294 \uc704\uc5d0 \uc5b8\uae09\ub41c \uc138 \ub2e8\uacc4\ub97c \ubc18\ubcf5\uc801\uc73c\ub85c \uc218\ud589\ud558\ub3c4\ub85d \uc124\uacc4\ub41c \uae30\uae30\uc785\ub2c8\ub2e4. \uac01 \ub2e8\uacc4\ub294 DNA \uc774\uc911 \ub098\uc120\uc758 \ud480\ub9bc, \ud504\ub77c\uc774\uba38\uc758 \uacb0\ud569 \ubc0f DNA \ubcf5\uc81c\ub97c \ud3ec\ud568\ud569\ub2c8\ub2e4. \uc5f4 \uc21c\ud658 \uc7a5\uce58\ub294 \uc628\ub3c4\ub97c \uc815\ubc00\ud558\uac8c \uc81c\uc5b4\ud558\uc5ec \uac01 \ub2e8\uacc4\uac00 \uc815\ud655\ud558\uac8c \uc218\ud589\ub418\ub3c4\ub85d \ud569\ub2c8\ub2e4.<\/p>\n

    1. \ubcc0\uc131 (Denaturation)<\/h3>\n

    \ubcc0\uc131 \ub2e8\uacc4\uc5d0\uc11c \uc5f4 \uc21c\ud658 \uc7a5\uce58\ub294 \uc0d8\ud50c\uc744 94-98\u00b0C\ub85c \uac00\uc5f4\ud558\uc5ec DNA \uc774\uc911 \ub098\uc120\uc744 \ub2e8\uc77c \uac00\ub2e5\uc73c\ub85c \ubd84\ub9ac\ud569\ub2c8\ub2e4. \uc774 \uacfc\uc815\uc740 \uc77c\ubc18\uc801\uc73c\ub85c 20-30\ucd08 \ub3d9\uc548 \uc9c0\uc18d\ub429\ub2c8\ub2e4.<\/p>\n

    2. \uc811\ud569 (Annealing)<\/h3>\n

    \uc811\ud569 \ub2e8\uacc4\uc5d0\uc11c \uc5f4 \uc21c\ud658 \uc7a5\uce58\ub294 \uc628\ub3c4\ub97c 50-65\u00b0C\ub85c \ub0ae\ucda5\ub2c8\ub2e4. \uc774 \uc628\ub3c4\uc5d0\uc11c \ud504\ub77c\uc774\uba38\uac00 \ub2e8\uc77c \uac00\ub2e5 DNA\uc5d0 \uacb0\ud569\ub429\ub2c8\ub2e4. \uc811\ud569 \ub2e8\uacc4\uc758 \uc9c0\uc18d \uc2dc\uac04\uc740 \ubcf4\ud1b5 20-40\ucd08\uc785\ub2c8\ub2e4.<\/p>\n

    3. \uc2e0\uc7a5 (Extension)<\/h3>\n

    \uc2e0\uc7a5 \ub2e8\uacc4\uc5d0\uc11c \uc5f4 \uc21c\ud658 \uc7a5\uce58\ub294 \uc628\ub3c4\ub97c 72\u00b0C\ub85c \uc62c\ub9bd\ub2c8\ub2e4. \uc774 \ub2e8\uacc4\uc5d0\uc11c Taq \uc911\ud569\ud6a8\uc18c\uac00 DNA \uac00\ub2e5\uc744 \ubcf5\uc81c\ud569\ub2c8\ub2e4. \uc2e0\uc7a5 \uc2dc\uac04\uc740 \ubcf4\ud1b5 1\ubd84 \uc815\ub3c4\uc785\ub2c8\ub2e4.<\/p>\n

    \uc628\ub3c4\uc758 \uc815\ubc00 \uc81c\uc5b4<\/h2>\n

    \uc5f4 \uc21c\ud658 \uc7a5\uce58\ub294 \uc138 \ub2e8\uacc4 \ub3d9\uc548 \uc628\ub3c4\ub97c \uc815\ubc00\ud558\uac8c \uc81c\uc5b4\ud560 \uc218 \uc788\uc5b4\uc57c \ud569\ub2c8\ub2e4. \uc2e4\ud5d8\uc758 \uc815\ud655\uc131\uc740 \uc774 \uc628\ub3c4 \uc81c\uc5b4\uc5d0 \ud06c\uac8c \uc758\uc874\ud569\ub2c8\ub2e4. \uc798\ubabb\ub41c \uc628\ub3c4 \uc81c\uc5b4\ub294 PCR \uacb0\uacfc\uc5d0 \ud070 \uc601\ud5a5\uc744 \ubbf8\uce60 \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/p>\n

    \uc5f4 \uc21c\ud658 \ud504\ub85c\uc138\uc2a4\uc758 \ubc18\ubcf5<\/h2>\n

    PCR \uacfc\uc815\uc740 \uc704\uc758 \uc138 \ub2e8\uacc4\ub97c 20-40\ud68c \ubc18\ubcf5\ud569\ub2c8\ub2e4. \uc774\ub97c \ud1b5\ud574 \uc218\ubc31\ub9cc \uac1c\uc758 DNA \ubcf5\uc81c\ubcf8\uc744 \uc5bb\uc744 \uc218 \uc788\uc2b5\ub2c8\ub2e4. \uc5f4 \uc21c\ud658 \uc7a5\uce58\ub294 \uc774\ub7ec\ud55c \ubc18\ubcf5 \uacfc\uc815\uc744 \ubb34\ub9ac \uc5c6\uc774 \uc218\ud589\ud560 \uc218 \uc788\ub3c4\ub85d \uc124\uacc4\ub418\uc5b4 \uc788\uc2b5\ub2c8\ub2e4.<\/p>\n

    \uacb0\ub860<\/h2>\n

    PCR\uc774 \uc131\uacf5\uc801\uc73c\ub85c \uc218\ud589\ub418\uae30 \uc704\ud574\uc11c\ub294 \uc5f4 \uc21c\ud658 \uc7a5\uce58\uc758 \uc815\ubc00\ud55c \uc628\ub3c4 \uc81c\uc5b4\uac00 \ud544\uc218\uc801\uc785\ub2c8\ub2e4. \uc774 \uc7a5\uce58\ub294 DNA \ubcf5\uc81c\uc758 \uac01 \ub2e8\uacc4\ub97c \uc815\ud655\ud558\uac8c \uc218\ud589\ud558\ub3c4\ub85d \ub3c4\uc640 \uc911\uc694 \uc5ed\ud560\uc744 \ud569\ub2c8\ub2e4. \uc5f4 \uc21c\ud658 \uc7a5\uce58 \ub355\ubd84\uc5d0 PCR\uc740 \uc720\uc804\uc790 \uc5f0\uad6c\uc640 \uc9c4\ub2e8\uc5d0\uc11c \ub110\ub9ac \uc0ac\uc6a9\ub418\uace0 \uc788\uc2b5\ub2c8\ub2e4.<\/p>\n

    \uc774\uc0c1\uc73c\ub85c \uc5f4 \uc21c\ud658 \uc7a5\uce58\uac00 PCR\uc744 \uc218\ud589\ud558\ub294 \ubc29\ubc95\uc5d0 \ub300\ud574 \uc54c\uc544\ubcf4\uc558\uc2b5\ub2c8\ub2e4. \uc774\ub7ec\ud55c \uae30\uc220\uc744 \uc774\ud574\ud568\uc73c\ub85c\uc368 \ubcf4\ub2e4 \uae4a\uc740 \uc0dd\ubb3c\ud559\uc801 \ubc0f \uc758\ud559\uc801 \uc5f0\uad6c\uac00 \uac00\ub2a5\ud574\uc9c8 \uac83\uc785\ub2c8\ub2e4.<\/p>\n","protected":false},"excerpt":{"rendered":"

    \uc5f4 \uc21c\ud658 \uc7a5\uce58\ub294 PCR \uc218\ud589 \uacfc\uc815\uc5d0\uc11c DNA \ubcf5\uc81c\ub97c \uc704\ud574 \uc138 \ub2e8\uacc4 \uc628\ub3c4 \ubcc0\ud654\ub97c \uc815\ubc00\ud558\uac8c \uc81c\uc5b4\ud558\ub294 \uc911\uc694\ud55c \uc2e4\ud5d8 \uae30\uae30\uc785\ub2c8\ub2e4.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[124],"tags":[],"yoast_head":"\n\uc5f4 \uc21c\ud658 \uc7a5\uce58\uac00 PCR\uc744 \uc218\ud589\ud558\ub294 \ubc29\ubc95<\/title>\n<meta name=\"description\" content=\"\uc5f4 \uc21c\ud658 \uc7a5\uce58\ub294 PCR \uc218\ud589 \uacfc\uc815\uc5d0\uc11c DNA \ubcf5\uc81c\ub97c \uc704\ud574 \uc138 \ub2e8\uacc4 \uc628\ub3c4 \ubcc0\ud654\ub97c \uc815\ubc00\ud558\uac8c \uc81c\uc5b4\ud558\ub294 \uc911\uc694\ud55c \uc2e4\ud5d8 \uae30\uae30\uc785\ub2c8\ub2e4.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, 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