{"id":362884,"date":"2024-06-22T12:19:18","date_gmt":"2024-06-22T11:19:18","guid":{"rendered":"https:\/\/www.thermal-engineering.org\/%ec%97%b4-%ec%88%9c%ed%99%98%ea%b8%b0-pcr-%ec%9c%a0%ed%98%95-%eb%b0%8f-%ec%8b%a4%ed%97%98%ec%8b%a4-%ec%9d%91%ec%9a%a9\/"},"modified":"2024-06-22T12:19:18","modified_gmt":"2024-06-22T11:19:18","slug":"%ec%97%b4-%ec%88%9c%ed%99%98%ea%b8%b0-pcr-%ec%9c%a0%ed%98%95-%eb%b0%8f-%ec%8b%a4%ed%97%98%ec%8b%a4-%ec%9d%91%ec%9a%a9","status":"publish","type":"post","link":"https:\/\/www.thermal-engineering.org\/ko\/%ec%97%b4-%ec%88%9c%ed%99%98%ea%b8%b0-pcr-%ec%9c%a0%ed%98%95-%eb%b0%8f-%ec%8b%a4%ed%97%98%ec%8b%a4-%ec%9d%91%ec%9a%a9\/","title":{"rendered":"\uc5f4 \uc21c\ud658\uae30 | PCR \uc720\ud615 \ubc0f \uc2e4\ud5d8\uc2e4 \uc751\uc6a9"},"content":{"rendered":"<p class=\"sidekick\">\uc5f4 \uc21c\ud658\uae30 (Thermal Cycler)\uc758 \ub2e4\uc591\ud55c \uc720\ud615\uacfc \uc2e4\ud5d8\uc2e4 \uc751\uc6a9 \ubd84\uc57c\uc5d0 \ub300\ud574 \uc27d\uac8c \uc124\uba85\ud569\ub2c8\ub2e4. PCR \uae30\uc220\uacfc DNA \uc99d\ud3ed\uc758 \ud575\uc2ec \uc6d0\ub9ac\ub97c \uc54c\uc544\ubcf4\uc138\uc694.<\/p>\n<p><img src=\"https:\/\/www.thermal-engineering.org\/wp-content\/uploads\/2024\/06\/\uc5f4_\uc21c\ud658\uae30_PCR_\uc720\ud615_\ubc0f_\uc2e4\ud5d8\uc2e4_\uc751\uc6a9.png\" alt=\"\uc5f4 \uc21c\ud658\uae30 | PCR \uc720\ud615 \ubc0f \uc2e4\ud5d8\uc2e4 \uc751\uc6a9\" style=\"display: block; margin-left: auto; margin-right: auto;\"\/><\/p>\n<h2>\uc5f4 \uc21c\ud658\uae30 (PCR)\uc758 \uc720\ud615 \ubc0f \uc2e4\ud5d8\uc2e4 \uc751\uc6a9<\/h2>\n<p>\uc5f4 \uc21c\ud658\uae30(Thermal Cycler)\ub294 \ud754\ud788 PCR (Polymerase Chain Reaction)\uc744 \uc2e4\ud589\ud558\ub294 \uc7a5\ube44\ub85c, \uc0dd\ubb3c\ud559\uacfc \uc0dd\uba85\uacf5\ud559 \uc5f0\uad6c\uc5d0\uc11c \ub9e4\uc6b0 \uc911\uc694\ud55c \uc5ed\ud560\uc744 \ud569\ub2c8\ub2e4. \uc774 \uc7a5\uce58\ub294 \uc628\ub3c4\ub97c \ubc18\ubcf5\uc801\uc73c\ub85c \ubcc0\ud654\uc2dc\ucf1c DNA\ub97c \uc99d\ud3ed\ud558\ub294 \uacfc\uc815\uc744 \uc790\ub3d9\ud654\ud569\ub2c8\ub2e4. \ubcf8 \uae00\uc5d0\uc11c\ub294 \uc5f4 \uc21c\ud658\uae30\uc758 \uc5ec\ub7ec \uc720\ud615\uacfc \uc2e4\ud5d8\uc2e4\uc5d0\uc11c\uc758 \uc751\uc6a9\uc5d0 \ub300\ud574 \uc54c\uc544\ubcf4\uaca0\uc2b5\ub2c8\ub2e4.<\/p>\n<h2>PCR\uc758 \uae30\ubcf8 \uc6d0\ub9ac<\/h2>\n<p>PCR\uc740 \ud2b9\uc815 DNA \uc870\uac01\uc744 \uc218\ubc31\ub9cc \ubc30 \uc774\uc0c1 \uc99d\ud3ed\ud558\ub294 \uae30\uc220\uc785\ub2c8\ub2e4. \uc774 \uacfc\uc815\uc5d0\ub294 \ub2e4\uc74c\uc758 \uc138 \uac00\uc9c0 \uc8fc\uc694 \ub2e8\uacc4\uac00 \ud3ec\ud568\ub429\ub2c8\ub2e4:<\/p>\n<ul>\n<li>\ubcc0\uc131(denaturation): \ub192\uc740 \uc628\ub3c4\uc5d0\uc11c \uc774\uc911 \ub098\uc120 \uad6c\uc870\uc758 DNA\uac00 \ub2e8\uc77c \uac00\ub2e5\uc73c\ub85c \ubd84\ub9ac\ub429\ub2c8\ub2e4.<\/li>\n<li>\ud504\ub77c\uc774\uba38 \uacb0\ud569(annealing): \uc628\ub3c4\uac00 \ub0ae\uc544\uc9c0\uba74\uc11c \ud504\ub77c\uc774\uba38\uac00 \ub2e8\uc77c \uac00\ub2e5 DNA\uc5d0 \uacb0\ud569\ud569\ub2c8\ub2e4.<\/li>\n<li>\ud655\uc7a5(extension): \uc57d\uac04 \ub192\uc740 \uc628\ub3c4\uc5d0\uc11c DNA \uc911\ud569\ud6a8\uc18c\uac00 \ud504\ub77c\uc774\uba38\uc5d0 \uacb0\ud569\ub41c DNA \ubd80\ubd84\uc744 \ubcf5\uc81c\ud558\uc5ec \uc0c8\ub85c\uc6b4 DNA \uac00\ub2e5\uc744 \ub9cc\ub4ed\ub2c8\ub2e4.<\/li>\n<\/ul>\n<h2>\uc5f4 \uc21c\ud658\uae30\uc758 \uc720\ud615<\/h2>\n<p>\uc5f4 \uc21c\ud658\uae30\ub294 \uc8fc\ub85c \ub2e4\uc74c\uacfc \uac19\uc774 \ubd84\ub958\ub429\ub2c8\ub2e4:<\/p>\n<ol>\n<li><b>\ud45c\uc900 \uc5f4 \uc21c\ud658\uae30<\/b>: \uac00\uc7a5 \uc77c\ubc18\uc801\uc73c\ub85c \uc0ac\uc6a9\ub418\ub294 \uc720\ud615\uc73c\ub85c, \ub2e4\uc591\ud55c \uc628\ub3c4\uc640 \uc2dc\uac04 \uc124\uc815\uc774 \uac00\ub2a5\ud569\ub2c8\ub2e4.<\/li>\n<li><b>\uc2e4\uc2dc\uac04 \uc5f4 \uc21c\ud658\uae30<\/b>: \uc2e4\uc2dc\uac04\uc73c\ub85c \ub370\uc774\ud130 \uc218\uc9d1\uc774 \uac00\ub2a5\ud558\uba70, \uc8fc\ub85c qPCR (quantitative PCR)\uc5d0 \uc0ac\uc6a9\ub429\ub2c8\ub2e4.<\/li>\n<li><b>\ub514\uc9c0\ud138 \uc5f4 \uc21c\ud658\uae30<\/b>: \ub9e4\uc6b0 \uc815\ubc00\ud558\uac8c \uc628\ub3c4\ub97c \uc81c\uc5b4\ud560 \uc218 \uc788\uc73c\uba70, \ud45c\uc900 PCR\ubcf4\ub2e4 \ub354 \uc815\ud655\ud55c \uacb0\uacfc\ub97c \uc81c\uacf5\ud569\ub2c8\ub2e4.<\/li>\n<\/ol>\n<h2>\uc2e4\ud5d8\uc2e4 \uc751\uc6a9<\/h2>\n<p>\uc5f4 \uc21c\ud658\uae30\ub294 \ub2e4\uc591\ud55c \uc2e4\ud5d8\uc2e4 \uc751\uc6a9 \ubd84\uc57c\uc5d0\uc11c \uc0ac\uc6a9\ub429\ub2c8\ub2e4. \uba87 \uac00\uc9c0 \uc8fc\uc694 \uc751\uc6a9 \ubd84\uc57c\ub294 \ub2e4\uc74c\uacfc \uac19\uc2b5\ub2c8\ub2e4:<\/p>\n<ul>\n<li><b>\uc720\uc804 \uc5f0\uad6c<\/b>: \uc720\uc804\uc790\ud615 \ubd84\uc11d \ubc0f \uc720\uc804\uc790 \ud074\ub85c\ub2dd\uc5d0 \uc0ac\uc6a9\ub429\ub2c8\ub2e4.<\/li>\n<li><b>\uc758\ub8cc \uc9c4\ub2e8<\/b>: \uac10\uc5fc\uc131 \uc9c8\ud658\uc758 \uc2e0\uc18d\ud55c \uc9c4\ub2e8, \uc608\ub97c \ub4e4\uc5b4 COVID-19 \ubc14\uc774\ub7ec\uc2a4 \uac80\ucd9c\uc5d0 \uc0ac\uc6a9\ub429\ub2c8\ub2e4.<\/li>\n<li><b>\ubc95\uc758\ud559<\/b>: DNA \uc9c0\ubb38 \ubd84\uc11d\uc744 \ud1b5\ud574 \ubc94\uc8c4 \ud604\uc7a5\uc5d0\uc11c \uc5bb\uc740 \uc99d\uac70\ub97c \ubd84\uc11d\ud558\ub294 \ub370 \uc0ac\uc6a9\ub429\ub2c8\ub2e4.<\/li>\n<li><b>\ub18d\uc5c5<\/b>: \uc791\ubb3c \ud488\uc885\uc758 \uc720\uc804\uc790 \ubcc0\ud615 \ubc0f \ud488\uc885 \uac1c\uc120 \uc5f0\uad6c\uc5d0 \uc0ac\uc6a9\ub429\ub2c8\ub2e4.<\/li>\n<\/ul>\n<h2>\uacb0\ub860<\/h2>\n<p>\uc5f4 \uc21c\ud658\uae30\ub294 \ud604\ub300 \uc0dd\ubb3c\ud559 \ubc0f \uc0dd\uba85\uacf5\ud559 \uc5f0\uad6c\uc5d0\uc11c \uc5c6\uc5b4\uc11c\ub294 \uc548 \ub420 \ub3c4\uad6c\uc785\ub2c8\ub2e4. PCR \uae30\uc220\uacfc \uc5f4 \uc21c\ud658\uae30\ub97c \ud1b5\ud574 \uacfc\ud559\uc790\ub4e4\uc740 \uc720\uc804 \uc815\ubcf4\ub97c \ube60\ub974\uace0 \uc815\ud655\ud558\uac8c \ubd84\uc11d\ud560 \uc218 \uc788\uc73c\uba70, \uc774\ub294 \ub9ce\uc740 \uc5f0\uad6c \ubc0f \uc2e4\ud5d8\uc2e4 \uc751\uc6a9 \ubd84\uc57c\uc5d0\uc11c \uc911\uc694\ud55c \uc5ed\ud560\uc744 \ud569\ub2c8\ub2e4. \uc9c0\uc18d\uc801\uc778 \uae30\uc220 \ubc1c\uc804\uacfc \ud568\uaed8 \uc5f4 \uc21c\ud658\uae30\ub294 \uc55e\uc73c\ub85c \ub354\uc6b1 \ub2e4\uc591\ud55c \ubd84\uc57c\uc5d0\uc11c \ud65c\uc6a9\ub420 \uac83\uc785\ub2c8\ub2e4.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\uc5f4 \uc21c\ud658\uae30 (Thermal Cycler)\uc758 \ub2e4\uc591\ud55c \uc720\ud615\uacfc \uc2e4\ud5d8\uc2e4 \uc751\uc6a9 \ubd84\uc57c\uc5d0 \ub300\ud574 \uc27d\uac8c \uc124\uba85\ud569\ub2c8\ub2e4. PCR \uae30\uc220\uacfc DNA \uc99d\ud3ed\uc758 \ud575\uc2ec \uc6d0\ub9ac\ub97c \uc54c\uc544\ubcf4\uc138\uc694.<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[124],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v15.4 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>\uc5f4 \uc21c\ud658\uae30 | PCR \uc720\ud615 \ubc0f \uc2e4\ud5d8\uc2e4 \uc751\uc6a9<\/title>\n<meta name=\"description\" content=\"\uc5f4 \uc21c\ud658\uae30 (Thermal Cycler)\uc758 \ub2e4\uc591\ud55c \uc720\ud615\uacfc \uc2e4\ud5d8\uc2e4 \uc751\uc6a9 \ubd84\uc57c\uc5d0 \ub300\ud574 \uc27d\uac8c \uc124\uba85\ud569\ub2c8\ub2e4. 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