{"id":728269,"date":"2024-11-27T21:03:57","date_gmt":"2024-11-27T20:03:57","guid":{"rendered":"https:\/\/www.thermal-engineering.org\/?p=728269"},"modified":"2024-11-27T21:03:57","modified_gmt":"2024-11-27T20:03:57","slug":"%e7%94%9f%e7%89%a9%e6%8a%80%e6%9c%af%e8%81%9a%e5%90%88%e9%85%b6%e9%93%be%e5%8f%8d%e5%ba%94%e4%b8%ad6%e7%a7%8d%e7%83%ad%e5%be%aa%e7%8e%af%e4%bb%aa%e7%b1%bb%e5%9e%8b%e8%a7%a3%e6%9e%90","status":"publish","type":"post","link":"https:\/\/www.thermal-engineering.org\/zh-hans\/%e7%94%9f%e7%89%a9%e6%8a%80%e6%9c%af%e8%81%9a%e5%90%88%e9%85%b6%e9%93%be%e5%8f%8d%e5%ba%94%e4%b8%ad6%e7%a7%8d%e7%83%ad%e5%be%aa%e7%8e%af%e4%bb%aa%e7%b1%bb%e5%9e%8b%e8%a7%a3%e6%9e%90\/","title":{"rendered":"\u751f\u7269\u6280\u672f\u805a\u5408\u9176\u94fe\u53cd\u5e94\u4e2d6\u79cd\u70ed\u5faa\u73af\u4eea\u7c7b\u578b\u89e3\u6790"},"content":{"rendered":"<p class=\"sidekick\">\u751f\u7269\u6280\u672f\u805a\u5408\u9176\u94fe\u53cd\u5e94\u4e2d6\u79cd\u70ed\u5faa\u73af\u4eea\u7c7b\u578b\u89e3\u6790\uff0c\u8be6\u89e3\u5404\u7c7b\u70ed\u5faa\u73af\u4eea\u7684\u5de5\u4f5c\u539f\u7406\u3001\u4f18\u7f3a\u70b9\u53ca\u5e94\u7528\u573a\u666f\uff0c\u5e2e\u52a9\u9009\u62e9\u6700\u5408\u9002\u7684\u8bbe\u5907\u3002<\/p>\n<p><img src=\"https:\/\/www.thermal-engineering.org\/wp-content\/uploads\/2024\/07\/\u751f\u7269\u6280\u672f\u805a\u5408\u9176\u94fe\u53cd\u5e94\u4e2d6\u79cd\u70ed\u5faa\u73af\u4eea\u7c7b\u578b\u89e3\u6790.png\" alt=\"\u751f\u7269\u6280\u672f\u805a\u5408\u9176\u94fe\u53cd\u5e94\u4e2d6\u79cd\u70ed\u5faa\u73af\u4eea\u7c7b\u578b\u89e3\u6790\" style=\"display: block; margin-left: auto; margin-right: auto;\"\/><\/p>\n<h2>\u751f\u7269\u6280\u672f\u805a\u5408\u9176\u94fe\u53cd\u5e94\u4e2d6\u79cd\u70ed\u5faa\u73af\u4eea\u7c7b\u578b\u89e3\u6790<\/h2>\n<p>\u805a\u5408\u9176\u94fe\u53cd\u5e94 (Polymerase Chain Reaction, PCR) \u662f\u751f\u7269\u6280\u672f\u4e2d\u7684\u4e00\u79cd\u57fa\u7840\u6280\u672f\uff0c\u7528\u4e8e\u6269\u589e\u7279\u5b9a\u7684DNA\u7247\u6bb5\u3002\u5728PCR\u53cd\u5e94\u8fc7\u7a0b\u4e2d\uff0c\u70ed\u5faa\u73af\u4eea (Thermal Cycler) \u662f\u5173\u952e\u8bbe\u5907\u3002\u672c\u6587\u5c06\u4ecb\u7ecd6\u79cd\u5e38\u89c1\u7684\u70ed\u5faa\u73af\u4eea\u7c7b\u578b\u3002<\/p>\n<p><u1><\/p>\n<li>\u4f20\u7edf\u70ed\u5faa\u73af\u4eea<\/li>\n<li>\u68af\u5ea6\u70ed\u5faa\u73af\u4eea<\/li>\n<li>\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u4eea<\/li>\n<li>\u5feb\u901f\u70ed\u5faa\u73af\u4eea<\/li>\n<li>\u591a\u5757\u70ed\u5faa\u73af\u4eea<\/li>\n<li>\u4fbf\u643a\u5f0f\u70ed\u5faa\u73af\u4eea<\/li>\n<p><\/u1><\/p>\n<h2>1. \u4f20\u7edf\u70ed\u5faa\u73af\u4eea<\/h2>\n<p>\u4f20\u7edf\u70ed\u5faa\u73af\u4eea\u662f\u6700\u57fa\u7840\u7684PCR\u8bbe\u5907\uff0c\u4e3b\u8981\u4f9d\u9760\u6e29\u63a7\u6a21\u7ec4\u5728\u4e0d\u540c\u6e29\u5ea6\u4e4b\u95f4\u5faa\u73af\u3002\u5b83\u4eec\u4e00\u822c\u5177\u6709\u7b80\u5355\u7684\u64cd\u4f5c\u754c\u9762\u548c\u7a33\u5b9a\u7684\u6027\u80fd\u3002<\/p>\n<h2>2. \u68af\u5ea6\u70ed\u5faa\u73af\u4eea<\/h2>\n<p>\u68af\u5ea6\u70ed\u5faa\u73af\u4eea\u5141\u8bb8\u5728\u540c\u4e00\u6b21PCR\u53cd\u5e94\u4e2d\u5bf9\u4e0d\u540c\u6e29\u5ea6\u533a\u95f4\u8fdb\u884c\u5b9e\u9a8c\u3002\u8fd9\u6837\uff0c\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u5728\u4e00\u6b21\u5b9e\u9a8c\u4e2d\u627e\u5230\u6700\u4f73\u9000\u706b\u6e29\u5ea6\uff0c\u63d0\u9ad8\u5b9e\u9a8c\u6548\u7387\u3002<\/p>\n<h2>3. \u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u4eea<\/h2>\n<p>\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u4eea\u4f7f\u7528\u8367\u5149\u67d3\u6599\u76d1\u6d4b\u548c\u91cf\u5316\u6269\u589e\u7684DNA\u7247\u6bb5\u3002\u5b83\u4eec\u9664\u4e86\u80fd\u5b8c\u6210\u5e38\u89c4\u7684PCR\u53cd\u5e94\u5916\uff0c\u8fd8\u80fd\u5728\u53cd\u5e94\u8fc7\u7a0b\u4e2d\u5b9e\u65f6\u68c0\u6d4b\u51faDNA\u7684\u91cf\u3002<\/p>\n<h2>4. \u5feb\u901f\u70ed\u5faa\u73af\u4eea<\/h2>\n<p>\u5feb\u901f\u70ed\u5faa\u73af\u4eea\u7684\u8bbe\u8ba1\u76ee\u6807\u662f\u7f29\u77edPCR\u53cd\u5e94\u65f6\u95f4\u3002\u5b83\u4eec\u901a\u5e38\u5177\u6709\u66f4\u8fc5\u901f\u7684\u5347\u964d\u6e29\u901f\u7387\uff0c\u53ef\u5728\u77ed\u65f6\u95f4\u5185\u5b8c\u6210DNA\u6269\u589e\uff0c\u9002\u7528\u4e8e\u5bf9\u65f6\u95f4\u8981\u6c42\u8f83\u9ad8\u7684\u7814\u7a76\u4e0e\u5e94\u7528\u3002<\/p>\n<h2>5. \u591a\u5757\u70ed\u5faa\u73af\u4eea<\/h2>\n<p>\u591a\u5757\u70ed\u5faa\u73af\u4eea\u642d\u8f7d\u591a\u4e2a\u52a0\u70ed\u6a21\u5757\uff0c\u53ef\u4ee5\u540c\u65f6\u8fdb\u884c\u591a\u7ec4\u4e0d\u540c\u6761\u4ef6\u7684PCR\u53cd\u5e94\u3002\u8fd9\u7c7b\u8bbe\u5907\u9002\u7528\u4e8e\u9700\u9ad8\u901a\u91cf\u3001\u9ad8\u6548\u7387\u8fdb\u884c\u5b9e\u9a8c\u7684\u5b9e\u9a8c\u5ba4\u3002<\/p>\n<h2>6. \u4fbf\u643a\u5f0f\u70ed\u5faa\u73af\u4eea<\/h2>\n<p>\u4fbf\u643a\u5f0f\u70ed\u5faa\u73af\u4eea\u5c0f\u5de7\u8f7b\u4fbf\uff0c\u53ef\u4ee5\u5728\u91ce\u5916\u6216\u8d44\u6e90\u6709\u9650\u7684\u73af\u5883\u4e2d\u4f7f\u7528\u3002\u5b83\u4eec\u901a\u5e38\u4f9d\u9760\u7535\u6c60\u4f9b\u7535\uff0c\u9002\u7528\u4e8e\u73b0\u573a\u5b9e\u9a8c\u548c\u5feb\u901f\u8bca\u65ad\u3002<\/p>\n<p>\u901a\u8fc7\u4e86\u89e3\u4e0d\u540c\u7c7b\u578b\u7684\u70ed\u5faa\u73af\u4eea\uff0c\u79d1\u7814\u4eba\u5458\u80fd\u591f\u9009\u62e9\u6700\u9002\u5408\u81ea\u5df1\u5b9e\u9a8c\u9700\u6c42\u7684\u8bbe\u5907\uff0c\u4ece\u800c\u63d0\u9ad8\u5b9e\u9a8c\u6548\u7387\u548c\u51c6\u786e\u6027\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u751f\u7269\u6280\u672f\u805a\u5408\u9176\u94fe\u53cd\u5e94\u4e2d6\u79cd\u70ed\u5faa\u73af\u4eea\u7c7b\u578b\u89e3\u6790\uff0c\u8be6\u89e3\u5404\u7c7b\u70ed\u5faa\u73af\u4eea\u7684\u5de5\u4f5c\u539f\u7406\u3001\u4f18\u7f3a\u70b9\u53ca\u5e94\u7528\u573a\u666f\uff0c\u5e2e\u52a9\u9009\u62e9\u6700\u5408\u9002\u7684\u8bbe\u5907\u3002<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[134],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v15.4 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>\u751f\u7269\u6280\u672f\u805a\u5408\u9176\u94fe\u53cd\u5e94\u4e2d6\u79cd\u70ed\u5faa\u73af\u4eea\u7c7b\u578b\u89e3\u6790<\/title>\n<meta name=\"description\" content=\"\u751f\u7269\u6280\u672f\u805a\u5408\u9176\u94fe\u53cd\u5e94\u4e2d6\u79cd\u70ed\u5faa\u73af\u4eea\u7c7b\u578b\u89e3\u6790\uff0c\u8be6\u89e3\u5404\u7c7b\u70ed\u5faa\u73af\u4eea\u7684\u5de5\u4f5c\u539f\u7406\u3001\u4f18\u7f3a\u70b9\u53ca\u5e94\u7528\u573a\u666f\uff0c\u5e2e\u52a9\u9009\u62e9\u6700\u5408\u9002\u7684\u8bbe\u5907\u3002\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermal-engineering.org\/zh-hans\/\u751f\u7269\u6280\u672f\u805a\u5408\u9176\u94fe\u53cd\u5e94\u4e2d6\u79cd\u70ed\u5faa\u73af\u4eea\u7c7b\u578b\u89e3\u6790\/\" \/>\n<meta property=\"og:locale\" content=\"zh_CN\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"\u751f\u7269\u6280\u672f\u805a\u5408\u9176\u94fe\u53cd\u5e94\u4e2d6\u79cd\u70ed\u5faa\u73af\u4eea\u7c7b\u578b\u89e3\u6790\" \/>\n<meta property=\"og:description\" content=\"\u751f\u7269\u6280\u672f\u805a\u5408\u9176\u94fe\u53cd\u5e94\u4e2d6\u79cd\u70ed\u5faa\u73af\u4eea\u7c7b\u578b\u89e3\u6790\uff0c\u8be6\u89e3\u5404\u7c7b\u70ed\u5faa\u73af\u4eea\u7684\u5de5\u4f5c\u539f\u7406\u3001\u4f18\u7f3a\u70b9\u53ca\u5e94\u7528\u573a\u666f\uff0c\u5e2e\u52a9\u9009\u62e9\u6700\u5408\u9002\u7684\u8bbe\u5907\u3002\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.thermal-engineering.org\/zh-hans\/\u751f\u7269\u6280\u672f\u805a\u5408\u9176\u94fe\u53cd\u5e94\u4e2d6\u79cd\u70ed\u5faa\u73af\u4eea\u7c7b\u578b\u89e3\u6790\/\" \/>\n<meta property=\"og:site_name\" content=\"Thermal Engineering\" \/>\n<meta property=\"article:published_time\" content=\"2024-11-27T20:03:57+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.thermal-engineering.org\/wp-content\/uploads\/2024\/07\/\u751f\u7269\u6280\u672f\u805a\u5408\u9176\u94fe\u53cd\u5e94\u4e2d6\u79cd\u70ed\u5faa\u73af\u4eea\u7c7b\u578b\u89e3\u6790.png\" \/>\n<meta name=\"twitter:card\" content=\"summary\" \/>\n<meta name=\"twitter:label1\" content=\"\u4f5c\u8005\">\n\t<meta name=\"twitter:data1\" content=\"Nick Connor\">\n\t<meta name=\"twitter:label2\" content=\"\u9884\u8ba1\u9605\u8bfb\u65f6\u95f4\">\n\t<meta name=\"twitter:data2\" content=\"0\u5206\">\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.thermal-engineering.org\/fr\/#website\",\"url\":\"https:\/\/www.thermal-engineering.org\/fr\/\",\"name\":\"Thermal Engineering\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":\"https:\/\/www.thermal-engineering.org\/fr\/?s={search_term_string}\",\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"zh-CN\"},{\"@type\":\"ImageObject\",\"@id\":\"https:\/\/www.thermal-engineering.org\/zh-hans\/%e7%94%9f%e7%89%a9%e6%8a%80%e6%9c%af%e8%81%9a%e5%90%88%e9%85%b6%e9%93%be%e5%8f%8d%e5%ba%94%e4%b8%ad6%e7%a7%8d%e7%83%ad%e5%be%aa%e7%8e%af%e4%bb%aa%e7%b1%bb%e5%9e%8b%e8%a7%a3%e6%9e%90\/#primaryimage\",\"inLanguage\":\"zh-CN\",\"url\":\"https:\/\/www.thermal-engineering.org\/wp-content\/uploads\/2024\/07\/\\u751f\\u7269\\u6280\\u672f\\u805a\\u5408\\u9176\\u94fe\\u53cd\\u5e94\\u4e2d6\\u79cd\\u70ed\\u5faa\\u73af\\u4eea\\u7c7b\\u578b\\u89e3\\u6790.png\",\"width\":1000,\"height\":1000,\"caption\":\"\\u751f\\u7269\\u6280\\u672f\\u805a\\u5408\\u9176\\u94fe\\u53cd\\u5e94\\u4e2d6\\u79cd\\u70ed\\u5faa\\u73af\\u4eea\\u7c7b\\u578b\\u89e3\\u6790\"},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.thermal-engineering.org\/zh-hans\/%e7%94%9f%e7%89%a9%e6%8a%80%e6%9c%af%e8%81%9a%e5%90%88%e9%85%b6%e9%93%be%e5%8f%8d%e5%ba%94%e4%b8%ad6%e7%a7%8d%e7%83%ad%e5%be%aa%e7%8e%af%e4%bb%aa%e7%b1%bb%e5%9e%8b%e8%a7%a3%e6%9e%90\/#webpage\",\"url\":\"https:\/\/www.thermal-engineering.org\/zh-hans\/%e7%94%9f%e7%89%a9%e6%8a%80%e6%9c%af%e8%81%9a%e5%90%88%e9%85%b6%e9%93%be%e5%8f%8d%e5%ba%94%e4%b8%ad6%e7%a7%8d%e7%83%ad%e5%be%aa%e7%8e%af%e4%bb%aa%e7%b1%bb%e5%9e%8b%e8%a7%a3%e6%9e%90\/\",\"name\":\"\\u751f\\u7269\\u6280\\u672f\\u805a\\u5408\\u9176\\u94fe\\u53cd\\u5e94\\u4e2d6\\u79cd\\u70ed\\u5faa\\u73af\\u4eea\\u7c7b\\u578b\\u89e3\\u6790\",\"isPartOf\":{\"@id\":\"https:\/\/www.thermal-engineering.org\/fr\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.thermal-engineering.org\/zh-hans\/%e7%94%9f%e7%89%a9%e6%8a%80%e6%9c%af%e8%81%9a%e5%90%88%e9%85%b6%e9%93%be%e5%8f%8d%e5%ba%94%e4%b8%ad6%e7%a7%8d%e7%83%ad%e5%be%aa%e7%8e%af%e4%bb%aa%e7%b1%bb%e5%9e%8b%e8%a7%a3%e6%9e%90\/#primaryimage\"},\"datePublished\":\"2024-11-27T20:03:57+00:00\",\"dateModified\":\"2024-11-27T20:03:57+00:00\",\"author\":{\"@id\":\"https:\/\/www.thermal-engineering.org\/fr\/#\/schema\/person\/e8c544db9afedaec8574d6464f9398bb\"},\"description\":\"\\u751f\\u7269\\u6280\\u672f\\u805a\\u5408\\u9176\\u94fe\\u53cd\\u5e94\\u4e2d6\\u79cd\\u70ed\\u5faa\\u73af\\u4eea\\u7c7b\\u578b\\u89e3\\u6790\\uff0c\\u8be6\\u89e3\\u5404\\u7c7b\\u70ed\\u5faa\\u73af\\u4eea\\u7684\\u5de5\\u4f5c\\u539f\\u7406\\u3001\\u4f18\\u7f3a\\u70b9\\u53ca\\u5e94\\u7528\\u573a\\u666f\\uff0c\\u5e2e\\u52a9\\u9009\\u62e9\\u6700\\u5408\\u9002\\u7684\\u8bbe\\u5907\\u3002\",\"inLanguage\":\"zh-CN\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.thermal-engineering.org\/zh-hans\/%e7%94%9f%e7%89%a9%e6%8a%80%e6%9c%af%e8%81%9a%e5%90%88%e9%85%b6%e9%93%be%e5%8f%8d%e5%ba%94%e4%b8%ad6%e7%a7%8d%e7%83%ad%e5%be%aa%e7%8e%af%e4%bb%aa%e7%b1%bb%e5%9e%8b%e8%a7%a3%e6%9e%90\/\"]}]},{\"@type\":\"Person\",\"@id\":\"https:\/\/www.thermal-engineering.org\/fr\/#\/schema\/person\/e8c544db9afedaec8574d6464f9398bb\",\"name\":\"Nick Connor\",\"image\":{\"@type\":\"ImageObject\",\"@id\":\"https:\/\/www.thermal-engineering.org\/fr\/#personlogo\",\"inLanguage\":\"zh-CN\",\"url\":\"https:\/\/secure.gravatar.com\/avatar\/84c0dec310b44b65da29dc9df6925239?s=96&d=mm&r=g\",\"caption\":\"Nick Connor\"}}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","_links":{"self":[{"href":"https:\/\/www.thermal-engineering.org\/zh-hans\/wp-json\/wp\/v2\/posts\/728269"}],"collection":[{"href":"https:\/\/www.thermal-engineering.org\/zh-hans\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.thermal-engineering.org\/zh-hans\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.thermal-engineering.org\/zh-hans\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.thermal-engineering.org\/zh-hans\/wp-json\/wp\/v2\/comments?post=728269"}],"version-history":[{"count":0,"href":"https:\/\/www.thermal-engineering.org\/zh-hans\/wp-json\/wp\/v2\/posts\/728269\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.thermal-engineering.org\/zh-hans\/wp-json\/wp\/v2\/media?parent=728269"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.thermal-engineering.org\/zh-hans\/wp-json\/wp\/v2\/categories?post=728269"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.thermal-engineering.org\/zh-hans\/wp-json\/wp\/v2\/tags?post=728269"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}